Fig. 5

Comparing cardiac myxoma (CM) tissues and the isolated cell line. a Micrograph showing exemplary histopathology findings of cardiac myxoma (CM), for patient ID 2 and ID 3 in H&E staining. CM cells stained in purple (blue arrow) can have stellate (ID 3) or globular (ID 2) shape. The red/orange structures (white arrow) correspond to the blood vessels with erythrocytes. The surrounding myxoid matrix is stained in pink (green arrow). b Confocal microscopy image with the CM cells stained for F-Actin (red), nucleus (blue), and VE-cadherin (green). The scale bar is 50 μm. c Micro-computed tomography results for CM (upper row) and adipose tissues (lower row). Histograms on the left side present normalized X-ray attenuation within the sample: (i) the mineral deposits range from 0.6 to 1.0 in the CM samples; (ii) the blood vessels range from 0.75 to 1.0 in the adipose tissue samples. These attenuation ranges have been binarized to extract the mineral deposits and blood vessels for further analysis and visualization. The right side contains volume-rendered 3-D models of the two most representative samples, namely CM and adipose tissues. The internal mineral deposits have been highlighted in the CM model. Its diameter has been color-coded using a heat map. The blood vessels have been colored red in the adipose tissue. d Results of the mean ortho-Positronium (o-Ps) lifetime for CM tissue (red circles), isolated from the same patient myxoma cell line (black squares). Patients ID annotations are described in Table 1