Fig. 2

Myxoma cell culture isolated from the tumor of patient ID 2 and ID 3. a (Left) The workflow of myxoma cell culture isolation. Cells have been isolated by tissue digestion and cultured to obtain the highest possible number of cells for the experiment. A micrograph presenting the primary culture of cells isolated from cardiac myxoma, upon seeding, and 24 h later with erythrocyte contamination. The latter has been washed out during the primary culture. Secondary culture after the first passage has been established after 1 week. A scale bar of 100 μm. b (Left) The workflow of PALS measurement. The centrifuged cells have been placed in both parts of the aluminum chamber with a radioactive source (red dot) encapsulated between them. The chamber has been mounted between detectors in the temperature-controlled aluminum holder. The measurements have been taken at 37 °C. (Right) Positronium lifetime spectra with fitted components for the myxoma cell culture isolated from the given patient. The dark yellow, green, turquoise, and blue lines denote direct annihilation in the source material, p-Ps annihilation component, direct annihilation in the sample, and o-Ps annihilation component, respectively. The spectra are shifted by the offset coming from the detection system configuration (~ 5 ns). The patient ID annotations are described in Table 1